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KMID : 0861020180330020045
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Korea Journal of Herbology
2018 Volume.33 No. 2 p.45 ~ p.52
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Protective effects of Smilacis Glabrae Rhizoma on acute pancreatitis
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Kim Myoung-Jin
Park Sung-Joo
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Abstract
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Objectives : Smilacis Glabrae Rhizoma (SGR) has been used in traditional medicine for treating diseases related to inflammation and cancer. However, the protective effect of SGR has not been reported in the study of acute pancreatitis (AP). Therefore, this study was performed to evaluate the protective effects of SGR water extract on cerulein-induced AP.
Methods : AP was induced by intraperitoneal injection of supramaximal concentrations of stable cholecystokinin analogue cerulein (50 ㎍/㎏) 6 times at intervals of one hour in mice. SGR water extract (0.01, 0.05, or 0.1 g/㎏) was administrated intraperitoneally 1 h prior to the first injection of cerulein. The mice were sacrificed at 6 h after the final injection of cerulein. Pancreas and lung were rapidly removed for histochemical examination and myeloperoxidase (MPO) assay. In addition, polymerase chain reaction (PCR) was performed to examine mRNA levels of proinflammatory cytokines such as interleukin(IL)-6, IL-1β and Tumor necrosis factor(TNF)-α.
Results : Administration of SGR water extract significantly inhibited pancreatic weight to body weight ratio. In addition, SGR treatment inhibited the histological damages and increase of MPO activity in both pancreas and lung during AP. Also, mRNA levels of IL-6 but not IL-1β and TNF-α were inhibited by SGR water extract against AP.
Conclusion : Our results revealed that pre-treatment of SGR water extract reduces the severity of cerulein-induced AP. Therefore, this study suggest that SGR could be used as a drug or agent to prevent AP.
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KEYWORD
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Acute pancreatitis, Cerulein, Cytokines, Pancreas, Smilacis Glabrae Rhizoma
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